| Authors: | S.Fan, C. Lin, x. zheng, N. Le Thomas | | Title: | UV Photonic integrated Circuits for Structured Illumination Microscopy with High Optical Throughput | | Format: | International Conference Poster | | Publication date: | 11/2023 | | Journal/Conference/Book: | Annual Symposium of the IEEE Photonics Society Benelux Chapter
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Abstract
Photonic integrated circuits (PICs) have the potential to upgrade the performances of standard optical microscopes. We have recently shown that PICs operating in the UV wavelength range enable label-free structured illumination microscopy (SIM) in a compact and robust way for biological imaging cells with improved spatial resolution. The state of the art for the field of view (FoV) of UV PICs-based SIM is currently 150 µm × 200 µm. This has been achieved by using a conventional imaging microscope objective, the numerical aperture of which was 0.5. The FoV is not limited by the imaging path but by the illumination, i.e. by the PIC design. Although a two-times resolution improvement is achieved, only a small fraction of the 1mm×1mm FoV offered by the objective, is used. Consequently, the optical throughput of the microscope is considerably limited, which hinders biological studies requiring the imaging of a large number of cells at the same time. To benefit from the maximum optical throughput, we present here a grating design for off-chip illumination at a wavelength of 360 nm with an 800 µm length. With a 2 nm shallow etched grating made of 5000 periods and patterned on an aluminum oxide waveguide, the FoV length is increased by four times. Related Research Topics
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